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PCR Enzymes and Kits


PCR mastermix                                              l  PCR Enzyme and Mastermix Catalog l  

ACTaq™ Mastermix

ACTaq™ Blue Mastermix

ACTaq™ Hot-Start Mastermix

ACTaq™ Blue Hot-Start Mastermix

ACTaq™ High Fidelity Mastermix

ACTaq™ Blue High Fidelity Mastermix


ACTaq™ Mastermix       P/N: E2110 

Dilution: 25μL of 2X ACTaq™ Mastermix is required for each 50μL reaction.

Product Description: ACTaq™ Mastermix is complete “ready-to-go” 2X reaction mix which only requires consumer researcher to add water, template and primers. Taq DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75ºC. Optimized for best performance when using dNTP concentration at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.

Storage Condition: -20ºC. Stable for 12 months in constant freezer temperature.

Unit Definition: One unit incorporates 10nmol dNTP into acid-insoluble material in 30
minutes at 72ºC.

Product Composition: 2.5 Units of ACTaq™ DNA Polymerase, 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl, 16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.

Quality Control: ACTaq™ DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.


 

ACTaq™ Blue Mastermix        P/N: E2110B (blue)

Dilution: 25μL of 2X ACTaq™ Mastermix is required for each 50μL reaction.

Product Description: ACTaq™ Blue / Red Mastermix is complete “ready-to-go” 2X reaction mix which only requires consumer researcher to add water, template and primers. ACTaq™ Blue / Red Mastermix differs from ACTaq™ Mastermix  such that it contains blue / red dye added to the enzyme used for tracking and loading dye purposes, and therefore no loading buffer is required. Taq ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75ºC. Optimized for best performance when using dNTP concentration at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.

Storage Condition: -20ºC. Stable for 12 months in constant freezer temperature.

Unit Definition: One unit incorporates 10nmol dNTP into acid-insoluble material in 30
minutes at 72ºC.

Product Composition: 2.5 Units of ACTaq™ Blue / Red Taq DNA Polymerase, 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl, 16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.

Quality Control: ACTaq™ Blue / Red Taq DNA Polymerase is highly purified. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.


 

ACTaq™ Hot-Start Mastermix  P/N: E3510

Dilution: 25μL of 2X ACTaq™ Hot-Start Mastermix is required for each 50μL reaction.

Product Description: ACTaq™ Hot-Start Mastermix is complete “ready-to-go” heat –activated 2X reaction mix which only requires the consumer researcher to add water, template and primers, and then pre-heat to 94ºC for reaction activation. It is one of most convenient way to set up PCR reaction. Not only it comes with the advantage of Hot-Start Taq enzyme, it goes further by including all
of the “ingredients” required for thermal cycling. Therefore, reduce the risk of contamination with ease and quick PCR setup, and ensuring greater reproducibility.

Hot-Start Activation Step: pre-incubate 2 minutes at 94ºC prior to thermal cycling.

Storage Condition: -20ºC. Stable for 12 months in constant freezer temperature.

Unit Definition: One unit of the amount of enzyme required to catalyze the incorporation of 1,000 nucleotides per minute at 65-72ºC.

Product Composition: 2.5 units of ACTaq™ HotStart DNA Polymerase (Antibody bound recombinant Taq DNA Polymerase); 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl,16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.

Quality Control: ACTaq™ Hot-Start DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.

 

ACTaq™ Blue Hot-Start Mastermix       P/N: E3510B (blue)

Dilution: 25μL of 2X ACTaq™ Blue / Red Hot-Start Mastermix is required for each 50μL reaction.

Product Description: ACTaq™ Blue / Red Hot-Start Mastermix is complete “ready-to-go” heat–activated 2X reaction mix which only requires the consumer researcher to add water, template and primers, and then pre-heat to 94ºC for reaction activation. It is one of most convenient way to set up PCR reaction. Not only it comes with the advantage of Hot-Start Taq enzyme, it goes further by including all of the “ingredients” required for thermal cycling and blue tracking and loading dye.

Hot-Start Activation Step: Pre-incubate 2 minutes at 94ºC prior to thermal cycling.

Storage Condition: -20ºC. Stable for 12 months in constant freezer temperature.

Unit Definition: One unit of the amount of enzyme required to catalyze the incorporation of 1,000 nucleotides per minute at 65-72ºC.

Product Composition: 2.5 units of ACTaq™ Blue / Red HotStart DNA Polymerase (Antibody bound recombinant Taq DNA Polymerase); 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl, 16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.

Quality Control: ACTaq™ Blue / Red Hot-Start DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.
 




ACTaq™ High Fidelity Mastermix       P/N: E2010

Dilution: 25μL of 2X ACTaq™ High Fidelity Mastermix is required for each 50μL reaction.

Product Description: ACTaq™ High Fideltiy Mastermix is complete “ready-to-go” reaction mix which only requires the consumer researcher to add water, template and primers. It is one of most convenient way to set up PCR reaction. Not only it comes with the advantage of High Fidelity Taq enzyme, it goes further by including all of the “ingredients” required for thermal cycling.

Storage Condition: -20ºC. Stable for 12 months in constant freezer temperature.

Unit Definition: One unit of the amount of enzyme incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72ºC.

Product Composition: 2.5 units of ACTaq™ High Fidelity DNA Polymerase; 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl, 16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.

Quality Control: ACTaq™ High Fideltiy DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.
   


ACTaq™ Blue High Fidelity Mastermix       P/N: E2010B (blue)

Dilution: 25μL of 2X ACTaq™ Blue High Fidelity Mastermix is required for each 50μL reaction.

Product Description: ACTaq™ Blue High Fideltiy Mastermix is complete “ready-to-go” 2X reaction mix which only requires the consumer researcher to add water, template and primers. It is one of most convenient way to set up PCR reaction. Not only it comes with the advantage of High Fidelity Taq enzyme, it goes further by including all of the “ingredients” required for thermal cycling and blue or red tracking and loading dye.

Storage Condition: -20ºC. Stable for 12 months in constant freezer temperature.

Unit Definition: One unit of the amount of enzyme incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72ºC.

Product Composition: 2.5 units of ACTaq™ Blue High Fidelity DNA Polymerase; 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl, 16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.

Quality Control: ACTaq™ High Fideltiy DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.
   




taq

 

 

PCR test results for ACTaq™ DNA Poly, ACTaq™ Blue DNA Poly, ACTaq™ Hot-Start DNA Poly, and ACTaq™ Mastermix

1    DNA Marker

2    ACTaq™ DNA Poly

3    ACTaq™ Blue DNA Poly

4    ACTaq™ Hot-Start DNA Poly

5    ACTaq™ Mastermix