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PCR Enzymes and Kits


Specialty PCR                                        l PCR Enzyme and Mastermix Catalog l 

ACTaq™ High Fidelity DNA Polymerase

ACTaq™ Blue High Fidelity DNA Polymerase

ACTaq™ Long DNA Polymerase

ACTaq™ Blue Long DNA Polymerase


ACTaq™ High Fidelity DNA Polymerase  P/N: E2000  

Product Description:           ACTaq™ High Fidelity  DNA Polymerase, originating from Pyrococcus furiosus with molecular weight of 90KDa, is a thermo stable enzyme and exhibits the lower error rate of DNA amplification.  For use in DNA fragment extension amplification reactions with 3' to 5' exonuclease  proofreading activity; at extension amplification speed of 1000 bases per minute and optimized between 65-75oC, when using dNTP concentration at 100-300μM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.1.  ACTaq™ High Fidelity DNA Polymerase generates blunt-ended fragment. 

Storage Condition:                  -20oC. Stable for at least 12 months in constant freezer temperature.   

Concentration:                          5 Units/μL

Unit Definition:                       One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.

Product Components:        ACTaq™ High Fidelity DNA Polymerase, 10x PCR reaction buffer with Mg2+
(Buffer and MgCl2 concentrations are customizable upon request) 

Storage Buffer:                       10mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol. 

PCR Reaction Buffer:            10x PCR buffer with Mg2+.  20mM MgSO4, 100mM KCl, 160mM
(NH4)2SO4,
, 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.
 

Quality Control:                      Activity of PCR for 1Kb fragment.  Free of contaminating endonucleases, exonucleases, and nicking activities.  The purity of the enzyme is evaluated by SDS-PAGE at >95% purity. 


ACTaq™ Blue High Fidelity DNA Polymerase

P/N: E2000B (blue)

Product Description:           ACTaq™ High Fidelity  DNA Polymerase, originating from Pyrococcus furiosus with molecular weight of 90KDa, is a thermo stable enzyme and exhibits the lower error rate of DNA amplification.  For use in DNA fragment extension amplification reactions with 3' to 5' exonuclease  proofreading activity; at extension amplification speed of 1000 bases per minute and optimized between 65-75oC, when using dNTP concentration at 100-300μM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.1. ACTaq™ High FidelityDNA Polymerase generates blunt-ended fragment. It comes with blue dye premixed with the enzyme used for tracking and loading dye purpose and therefore no additional loading buffer if needed.

Storage Condition:                  -20oC. Stable for at least 12 months in constant freezer temperature.   

Concentration:                            1 Unit/μL

Unit Definition:                       One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.

Product Components:        ACTaq™ Blue High Fidelity DNA Polymerase, 10x PCR reaction buffer with Mg2+
(Buffer and MgCl2 concentrations are customizable upon request) 

Storage Buffer:                       10mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol. 

PCR Reaction Buffer:            10x PCR buffer with Mg2+.  20mM MgSO4, 100mM KCl, 160mM
(NH4)2SO4,
, 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.
 

Quality Control:                      Activity of PCR for 1Kb fragment.  Free of contaminating endonucleases, exonucleases, and nicking activities.  The purity of the enzyme is evaluated by SDS-PAGE at >95% purity. 


 

ACTaq™ Long DNA Polymerase

Product Description:           ACTaq™ Long DNA Polymerase is a specialty taq for high processivity and fidelity at extension amplification speed fo 1000 bases per minute and optimized b etween 65-75oC, when using dNTP concentration at 100-500μM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.2. 

Storage Condition:                  -20oC. Stable for at least 12 months in constant freezer temperature.   

Concentration:                          5 Units/μL

Unit Definition:                       One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.

Product Components:        ACTaq™ Long DNA Polymerase, 10x PCR reaction buffer with Mg2+
(Buffer and MgCl2 concentrations are customizable upon request) 

Storage Buffer:                       10mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol. 

PCR Reaction Buffer:            10x PCR buffer with Mg2+.  20mM MgSO4, 100mM KCl, 160mM
(NH4)2SO4,
, 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.
 

Quality Control:                      ACTaq™ Long DNA Polymerase is highly purified.  Free of contaminating endonucleases, exonucleases, and nicking activities.  The purity of the enzyme is evaluated by SDS-PAGE at >95% purity. 


ACTaq™ Blue Long DNA Polymerase

Product Description:           ACTaq™ Long DNA Polymerase is a specialty taq for high processivity and fidelity at extension amplification speed fo 1000 bases per minute and optimized b etween 65-75oC, when using dNTP concentration at 100-500μM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.2. It comes with blue dye premixed with the enzyme used for tracking and loading dye purpose and therefore no additional loading buffer if needed.

Storage Condition:                  -20oC. Stable for at least 12 months in constant freezer temperature.   

Concentration:                            1 Unit/μL

Unit Definition:                       One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.

Product Components:        ACTaq™ Blue Long DNA Polymerase, 10x PCR reaction buffer with Mg2+
(Buffer and MgCl2 concentrations are customizable upon request) 

Storage Buffer:                       10mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol. 

PCR Reaction Buffer:            10x PCR buffer with Mg2+.  20mM MgSO4, 100mM KCl, 160mM
(NH4)2SO4,
, 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.
 

Quality Control:                      ACTaq™ Long DNA Polymerase is highly purified.  Free of contaminating endonucleases, exonucleases, and nicking activities.  The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.