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PCR Enzymes and Kits


Standard PCR                                        l PCR Enzyme and Mastermix Catalog l

ACTaq™ DNA Polymerase

ACTaq™ Blue DNA Polymerase

ACTaq™ Red DNA Polymerase


ACTaq™ DNA Polymerase           P/N:   E2100

Product Description: ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular
weight of 94KDa. For use in DNA fragment extension amplification
reactions with DNA having dA overhang on 3’ ends; at extension
amplification speed of 1200 bases per minute and optimized between
65-75oC. Optimized for best performance when using dNTP concentration
at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.

Storage Condition: -20oC. Stable for 12 months in constant freezer temperature.

Concentration: 5 Units/μL

Unit Definition: One unit of the amount of enzyme that incorporates 10nmols of dNTPs into
acid-insoluble material in 30 minutes at 72oC.

Product Components: ACTaq™ DNA Polymerase, PCR reaction buffer, 10X 25mM MgCl2. (Buffer and MgCl2 concentrations are customizable upon request)

Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol.

PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.

Quality Control: ACTaq™ DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.


 

ACTaq Blue DNA Polymerase                         

ACTaq™ Red DNA Polymerase    

P/N:E2100B (blue), E2100R (red)

P
roduct Description: ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular
weight of 94KDa.
 ACTaq™ Blue or Red
differs from ACTaq™ DNA Polymerase in that it contains blue / red dye added to the enzyme used as tracking and loading dye as no loading buffer is required.

Storage Condition: -20oC. Stable for 12 months in constant freezer temperature.

Concentration: 1 Unit/μL

Unit Definition: One unit of the amount of enzyme that incorporates 10nmols of dNTPs into
acid-insoluble material in 30 minutes at 72oC.

Product Components: ACTaq™ Blue or Red DNA Polymerase, PCR reaction buffer, 10X 25mM MgCl2.
(Buffer and MgCl2 concentrations are customizable upon request)

Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol.

PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.

Quality Control: ACTaq™ Blue or Red DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.

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PCR test results for ACTaq™ DNA Poly, ACTaq™ Blue DNA Poly, ACTaq™ Hot-Start DNA Poly, and ACTaq™ Mastermix

1    DNA Marker

2    ACTaq™ DNA Poly

3    ACTaq™ Blue DNA Poly

4    ACTaq™ Hot-Start DNA Poly

5    ACTaq™ Mastermix