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Transfection Reagent


Convoy™ Transfection Reagent                      l Product Literature l

G-418 (aminoglycosidic Antibiotic)


ConvoyTMTransfection Reagent   l Detail Transfection Protocol l

ConvoyTM is new generation cationic polymer gene transfection reagent. It has several unique features necessary for efficient transfection - DNA condensation and endosomal release, which improves gene transfection efficiency.

Compared with cationic lipids, cationic polymers are extremely stable, easy to handle, and more resistant to serum in cell culture.

The above advantages make gene transfection much easier and reproducible. ConvoyTM are widely used for both primary cell and established cell lines.

 

ConvoyTMTrantransfection picturesfection

 


Feature Highlights:

- High transfection efficiency
Under comparison with other commercial brands in the U.S., ConvoyTM is more stable both in presence and absence of serum, and shows higher transfection efficiency than cationic lipid in some most commonly used cell lines.
Convoy TM shows especially higher transfection efficiency in HUVEC (primary cell), which is insensate to most transfection reagents including cationic lipids. 

convoy efficiency

  • Figure 1: The pCMV-luc plasmid was transfected into HEK 293 cells and then assayed for luciferase activity 48 hours post-transfection.  Panel (a): Convoy™ Transfection Reagent; Panel (b): Competitor A transfection reagent: Panel (c): competitor B transfection reagent: Panel (d): Competitor C transfection reagent. 

 

 

convoy efficiency2

Figure 2: HUVEC cells transfection (100x magnification).Panel (a): Convoy™ Transfection Reagent; Panel (b): Competitor A transfection reagent; Panel (c): Competitor B trasnfection reagent; Panel (d): Competitor C transfection reagent

 

 

 

 convoy higher gene expression

Figure 3: HEK 293 cells were transfected with plasmid DNA containing lacZ gene.  Cells incorporating lacZ gene metabolize X-Gal and are stained blue.  Cells were assayed 48 hrs post transfection.  Panel (a): Convoy™ Transfection Reagent; Panel (b): Competitor A transfection reagent; Panel (c): Competitor B transfection reagent; Panel (d): Competitor C transfection reagent.

 

 

convoy more transfected cells

  • Figure 4: HEK 293 cells transfected with pEGFPN1 and shown 48 hrs post transfection (100x magnification). Panel (a): Convoy™ Transfection Reagent; Panel (b): Competitor A transfection reagent; Panel (c): Competitor B transfection reagent; Panel (d): Competitor C transfection reagent.
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- Low Cytotoxicity
The cell survival rate is over 90%, when experiment is carried in suitable condition and with recommended ConvoyTM dosage.

convoy cell viability

  • Figure 5: The viability of HEK 293 cells was assessed using MTT method 48 hrs post transfection.  Each transfection reagent was used under optimal conditions.  Panel (a): Convoy™ Transfection Reagent: Panel (b): Competitor A transfection reagent: Panel (c): Competitor B transfection reagent: Panel (d): Competitor C trasnfection reagent.


 

- Simplified Protocol < 30 minutes   l Detail Transfection Protocol l

As a serum resistant reagent, the transfection procedure is very simple: the DNA/transfection reagent complexes can be directly added into complete cell medium and waited for transfection assay (See Protocol Link above). With no need to change medium, the transfection can be finished in half an hour, and the whole procedure is simple, short and flexible. Additionally, cytotoxicity is found to be lower with ConvoyTM by carrying out simplified shortened protocol. 

 

   - Compatibility with most cell lines

convoy for all cell lines

Figure 6: The pCMB-luc plasmid was transfected into a number of cell lines.  The cells were then assayed for luciferase activity 48 hrs post transfection.  Panel (a): Convoy™ Transfection Reagent: Panel (b): Competitor A transfection reagent: Panel (C): Competitor B transfection reagent: Panel (d): Competitor C trasnfection reagent.

 

 

 

Cell lines (not limited to below) successfully transfected using Convoy™  

Cell line

Origin

Cell type

HEK 293

Human

Embryonic kidney

HeLa

Human

Cervix carcinoma

NIH 3T3

Mouse

Embryo fibroblast

BNL CL2

Mouse

Embryonic kidney cells

HepG2

Human

Hepatocarcinoma

COS7

Monkey

SV40   kidney    transformed

CHO

Chinese hamster

Ovary

5HSY-5Y

Human

Neuroblastoma

IMR 32

Human

Neuroblastoma

MRC5

Human

Fetal lung epithelium

MCF7

Human

Breast adenocarcinoma

K562

Human

Chronic leukemia

SKOV-3

Human

Ovarian adenocarcinoma

IGROV-1

Human

Ovarian adenocarcinoma

HUVEC (primary cell)

Human

Umbilical endothelial cell

Looking for more data? Please visit Detail Transfection Protocol or simply contact us.

 


 G-418 (aminoglycosidic Antibiotic) - suggested item for transfection experiments

G-418 sulfate
Aminoglycosidic antibiotic

White powder, shipped at room temperature, dry powder stored at RT and solution at -20°C.

Formula: C20H40N4O10¡¤2H2SO4 (CAS No.: 108321-42-2)

Molecular weight: 692.71

 
C20H40N4O10¡¤2H2SO4 (CAS No.: 108321-42-2) Molecular weight: 692.71
Purpose: Acts by interfering with protein synthesis and is usually used as an agent for selection of cultured cells expressing a gene that confers resistance to G-418.
Potency: 700 µg/mg. Min.
Working conc.: 150 µg/ml
Packaging:100mg, 500mg, 1g, 2g, 5g, 25g, 100g, 500g, 1 kg or any specified.
For research use only. Not for human or drug use.